10-layer cellstacks (Corning Life Sciences)
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Corning Life Sciences
10-layer cellstacks
10 Layer Cellstacks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10-layer+cellstacks/t+flasks/pmc12124627-181-104-106
Average 90 stars, based on 1 article reviews
10 Layer Cellstacks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10-layer+cellstacks/t+flasks/pmc12124627-181-104-106
Average 90 stars, based on 1 article reviews
10-layer cellstacks - by Bioz Stars,
2026-09
90/100 stars
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Recombinant:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Generated:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Homologous Recombination:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Plasmid Preparation:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Bioprocessing:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Expressing:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Functional Assay:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Produced:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Derivative Assay:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Stable Transfection:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using Infection:Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al. Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences. Article Snippet: Article Title: Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striking quantitative and qualitative differences Article Snippet: Production of Article Title: Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection Article Snippet: Recombinant herpes viruses rHSV-RC and rHSV-GOI (rHSV-CK8-μDys5) were generated by homologous recombination on the backbone of d27-1 , a replication-defective HSV vector according to a previously published method., Although replication defective, d27.1 does express the HSV early genes required for AAV replication and packaging. rHSV-RC was engineered to express AAV2 rep and AAV9 cap genes and rHSV-GOI contains GOI, consisting of AAV2 ITRs and a muscle-specific CK8 promoter to drive expression of a fully functional truncated dystrophin termed micro-dystrophin (μDys5). rHSV stocks were produced by infecting complementing V27 cells, a line derived from parental Vero cells to stably expresses HSV ICP27 , using the Article Title: Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions Article Snippet: Suspension cultures, in which human mesenchymal stem cells are cultivated on microcarriers in scalable single-use stirred bioreactor types, have been shown to be a promising alternative to planar flask cultures.. However, stirred single-use bioreactors were originally developed for production processes with robust, permanent cell lines.. Human mesenchymal stem cells are adherent primary cells and thus expanding them in such bioreactor systems imposes more stringent requirements on bioreactor systems. Article Title: AAV mediated influenza vaccines Article Snippet: Cells are cultivated in Article Title: NaCl and KCl mediate log increase in AAV vector particles and infectious titers in a specific/timely manner with the HSV platform Article Snippet: Helper rHSV-AAV9 contained the entire expression cassette of AAV2 Rep and AAV9 Cap under their respective endogenous promoters, as described in Adamson-Small et al.9 Production of rHSV-GFP and rHSV-AAV9 stocks was performed by infecting V27 cells in Article Title: Construction of stable packaging cell lines for clinical lentiviral vector production Article Snippet: Vector production was also scaled up to allow the collection of 640 ml or 560 ml per harvest using |